VEGFR3/Flt-4 Antibody [Unconjugated]

Images

 
VEGFR3/Flt‑4 was detected in immersion fixed paraffin-embedded sections of human cervical squamous metaplasia using Human VEGFR3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF349) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

VEGFR3/Flt-4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human VEGFR3/Flt-4
Tyr25-Ile776
Accession # P35916
Specificity
Detects human VEGFR3/Flt-4 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse VEGFR3 is observed and less than 2% cross-reactivity with recombinant human VEGFR1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT4
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF349 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR3/Flt-4 Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt-4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF349
Species: Hu
Applications: WB, IHC

Publications for VEGFR3/Flt-4 Antibody (AF349)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(11)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF349 Applications Species
LD Volk-Drape, KL Hall, AC Wilber, S Ran Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4 PLoS ONE, 2017;12(6):e0179257. 2017 [PMID: 28598999] (Flow Cytometry, Human) Flow Cytometry Human
K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling Circ. Res, 2017;0(0):. 2017 [PMID: 28298294] (Western Blot, Human) Western Blot Human
Tom J Parry Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction Eur. J. Pharmacol, 2016;796(0):76-89. 2016 [PMID: 27993643] (Western Blot, Human) Western Blot Human
Masaaki Miyazawa Specific niches for lung-resident memory CD8+ T cells at the site of tissue regeneration enable CD69-independent maintenance J. Exp. Med., 2016;0(0):. 2016 [PMID: 27815325] (IHC-Fr, Mouse) IHC-Fr Mouse
TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT) PLoS ONE, 2016;11(9):e0162221. 2016 [PMID: 27589056] (Western Blot, Human) Western Blot Human
Wakisaka N, Hasegawa Y, Yoshimoto S, Miura K, Shiotani A, Yokoyama J, Sugasawa M, Moriyama-Kita M, Endo K, Yoshizaki T Primary Tumor-Secreted Lymphangiogenic Factors Induce Pre-Metastatic Lymphvascular Niche Formation at Sentinel Lymph Nodes in Oral Squamous Cell Carcinoma. PLoS ONE, 2015;10(12):e0144056. 2015 [PMID: 26630663] (IHC-P, Human) IHC-P Human
Omoto, Itaru, Matsumoto, Masataka, Okumura, Hiroshi, Uchikado, Yasuto, Setoyama, Tetsuro, Kita, Yoshiaki, Owaki, Tetsuhir, Kijima, Yuko, Shinchi, Hiroyuki, Ishigami, Sumiya, Ueno, Shinichi, Natsugoe, Shoji Expression of vascular endothelial growth factor-C and vascular endothelial growth factor receptor-3 in esophageal squamous cell carcinoma. Oncol Lett, 2014;7(4):1027-1032. 2014 [PMID: 24944663] (IHC, Human) IHC Human
Thelen A, Scholz A, Benckert C, Von Marschall Z, Schroder M, Wiedenmann B, Neuhaus P, Rosewicz S, Jonas S VEGF-D promotes tumor growth and lymphatic spread in a mouse model of hepatocellular carcinoma. Int. J. Cancer, 2008;122(11):2471-81. 2008 [PMID: 18338756] (IHC, Human) IHC Human
Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma. Cancer Res., 2004;64(2):554-64. 2004 [PMID: 14744769] (Western Blot, Human) Western Blot Human
Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells. Leuk. Res., 2004;28(3):243-8. 2004 [PMID: 14687619] (ICC, Human) ICC Human
Show All 12 Publications.

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Secondary Antibodies

 

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Biotin BAF349

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Bioinformatics

Gene Symbol FLT4
Uniprot