VEGFR1/Flt-1 Antibody (611926) [Unconjugated] - Variant Flt1-14

Western blot shows human serum (preeclampsia). PVDF Membrane was probed with 2 µg/mL of Human VEGFR1/Flt-1 Variant Flt1-14 Monoclonal Antibody (Catalog # MAB6564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VEGFR1/Flt-1 Variant Flt1-14 at approximately 90 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 10.

Product Discontinued

VEGFR1/Flt-1 Antibody (611926) [Unconjugated] - Variant Flt1-14 Summary

• Immunogen: Human VEGFR1/Flt-1 Variant Flt1-14 synthetic peptide
  Glu706-Leu721
  Accession # ACA62948
• Specificity: Detects synthetic peptide (aa 706-721) from human VEGFR1/Flt-1 Variant Flt1-14 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (611926) [Unconjugated] - Variant Flt1-14

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

Human VEGFR1/Flt-1 is a type I transmembrane receptor for the angiogenic cytokine, VEGF. Alternate splicings of human VEGFR1 mRNA encode soluble, secreted isoforms that can function as inhibitors of VEGF. One, designated sFlt1-14 or sFlt1-e15a, encodes a 733 amino acid (aa), 95-135 kDa form that is produced by non-endothelial cells, notably by degenerative syncytiotrophoblasts within syncytial knots in the placenta. This form appears to be unique to primates and is produced in excess during preeclampsia of pregnancy. The peptide used as an immunogen is not present within either the full-length or alternate secreted forms of the protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • ACA62948()