Bovine VEGF-B ELISA Kit (Colorimetric)

• Reactivity: Bv
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 7.8 pg/mL (example only; lot dependent)

Bovine VEGF-B ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 7.8 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VEGFB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Bovine VEGF-B ELISA Kit (Colorimetric)

• vascular endothelial growth factor B
• VEGFB
• VEGF-B
• VEGF-related factor
• VRFVEGFL

Background

VEGFB 186 is an isoform of VEGFB (Vascular Endothelial Growth Factor B), a member of the vascular endothelial growth factor (VEGF) family. It is a growth factor for endothelial cells. Both isoforms of VEGFB show strong homology to VEGF at their amino termini, wheras the VEGFB167 isoform binds heparin, the C terminus of VEGFB-186 has no heparin binding domain, is less basic and more hydrophobic, and has no similarity to any published sequence. VEGFB 186 is secreted as a disulfide-linked homodimer and that, like VEGFB 167, this isoform can form disulfide-linked heterodimers with VEGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VEGF-B ELISA Kit (NBP3-53922). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VEGFB