VEGF-B Antibody (MM0008-7B43)

Immunohistochemistry-Paraffin: VEGFB Antibody (MM0008-7B43) [NB110-60974] - Formalin fixed and paraffin embedded normal human placenta tissue is subjected to mouse anti human VEGF-B.

Product Discontinued

VEGF-B Antibody (MM0008-7B43) Summary

• Immunogen: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human VEGF-B167 recombinant protein.
• Specificity: This antibody detects both human VEGF-B 167 and 186 isoforms. No crossreactivity is shown to hVEGF, VEGF-C and VEGF-D
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VEGFB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:100
  • Western Blot 1:500-1:1000
• Control:

  VEGF-B Overexpression Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.2 um filtered PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for VEGF-B Antibody (MM0008-7B43)

• vascular endothelial growth factor B
• VEGFB
• VEGF-B
• VEGF-related factor
• VRFVEGFL

Background

endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VEGFB
• Entrez:
  • 7423(Human)
• Uniprot:
  • P49765()