VDP p115 Antibody

Western Blot: VDP p115 Antibody [NB100-74483] - The Uso1 gene trap allele does not produce a functional polypeptide. Immunoblots of SDS-PAGE separated cell lysate extracted from wild-type, HET AW0562 GT and HET YTA025 GT fibroblasts. Left panel: an anti-USO1 antibody whose epitope is amino-terminal (N-term.) to the site of the USO1-Beta-Geo fusion detects full-length USO1 protein (arrow) in all lysates. No unique band representing a USO1-Beta-Geo fusion protein is observed in either heterozygous GT fibroblast lysate, although smaller bands of indeterminate origin are detected in wild-type and GT lysates. Right panel: an anti-USO1 antibody whose epitope is carboxyl-terminal (C-term.) to the site of the USO1-Beta-Geo fusion detects full length USO1 protein (arrow) in all lysates. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0050530) licensed under a CC-BY license.

Western Blot: VDP p115 Antibody [NB100-74483] - Analysis of rat liver.

The Uso1 gene trap allele does not produce a functional polypeptide. B) Immunoblots of SDS-PAGE separated cell lysate extracted from wild-type, HET AW0562 GT and HET YTA025 GT fibroblasts. Left panel: an anti-USO1 antibody whose epitope is amino-terminal (N-term.) to the site of the USO1-Beta-Geo fusion detects full-length USO1 protein (arrow) in all lysates. No unique band representing a USO1-Beta-Geo fusion protein is observed in either heterozygous GT fibroblast lysate, although smaller bands of indeterminate origin are detected in wild-type and GT lysates. Right panel: an anti-USO1 antibody whose epitope is carboxyl-terminal (C-term.) to the site of the USO1-Beta-Geo fusion detects full length USO1 protein (arrow) in all lysates. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0050530), licensed under a CC-BY licence.

Product Discontinued

VDP p115 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues M(1) N F L R G V M G G Q S A G P Q H(17) of rat p115.
• Localization: Peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase.
• Specificity: Rat VDP p115. The sequence is conserved across many species including mouse and human.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 3 - 5 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence 2 - 3 ug/mL
  • Western Blot 1:500
• Application Notes: Useful in WB. WB: Detects an approx. 115 kDa protein representing p115 in rat and mouse liver samples.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VDP p115 Antibody

• p115
• Transcytosis-associated protein
• USO1 homolog, vesicle docking protein (yeast)
• USO1 vesicle docking protein homolog (yeast)
• vesicle docking protein p115
• vesicle docking protein

Background

VDP p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VDP p115 Antibody (NB100-74483)(1)

Publication using NB100-74483

• Kim S, Hill A, Warman ML, Smits P. Golgi Disruption and Early Embryonic Lethality in Mice Lacking USO1 PLoS One 2012-01-01 [PMID: 23185636] (WB, Mouse)

Bioinformatics

• Gene Symbol: USO1
• Entrez:
  • 8615(Human)
  • 56041(Mouse)
  • 56042(Rat)
• Uniprot:
  • P41542(Rat)