VASP Antibody (SY02-16)

Western Blot: VASP Antibody (SY02-16) [NBP2-67443] - Analysis of VASP on different lysates using anti-VASP antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HT29

Product Discontinued

VASP Antibody (SY02-16) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human VASP aa 51-100 / 380. (SwissProt: P50552 Human)
• Localization: Cytoplasm, Cell junction, Cell projection.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VASP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for VASP Antibody (SY02-16)

• vasodilator-stimulated phosphoprotein
• VASP

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VASP Antibody (NBP2-67443)(1)

Publication using NBP2-67443

• Duan L, Schimmelmann M, Wu Y et al. CCN3 Signaling Is Differently Regulated in Placental Diseases Preeclampsia and Abnormally Invasive Placenta Frontiers in endocrinology 2020-11-16 [PMID: 33304321] (WB, Human)

Bioinformatics

• Gene Symbol: VASP