VAP-B Antibody (736904) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
736904
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

VAP-B Antibody (736904) [Alexa Fluor® 647] Summary

Immunogen
E. coli-derived recombinant human VAP-B
Ala2-Pro132
Accession # O95292
Specificity
Detects human VAP-B in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human VAP-A is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VAPB
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAP-B Antibody (736904) [Alexa Fluor® 647]

  • ALS8
  • ALS8VAMP-B/VAMP-C
  • VAMP (vesicle-associated membrane protein)-associated protein B and C
  • VAMP-associated 33 kDa protein
  • VAMP-associated protein B/C
  • VAMP-B
  • VAMP-C
  • VAPB
  • VAP-B
  • VAP-B/VAP-C
  • VAPC
  • VAP-C
  • vesicle-associated membrane protein-associated protein B/C

Background

Vesicle-associated membrane protein (VAMP)-associated protein B (VAP-B; also VAMP-B) is an ~30 kDa ubiquitously expressed type IV transmembrane protein belonging to the VAP family (1, 2). It is found in endoplasmic reticulum (ER), Golgi and other membranes as a homodimer or a heterodimer with VAP-A, probably associating through a GxxxG motif in the transmembrane regions (1, 2). Human VAP-B cDNA encodes 243 amino acids (aa) that include a 222 aa cytoplasmic domain and a 21 aa C-terminal membrane anchor. The cytoplasmic domain contains a mobile sperm protein (MSP) domain (aa 7‑124) and a coiled-coil region (aa 159‑196). Human VAP-B shares 90%, 89%, 96%, 96% and 94% aa identity with mouse, rat, canine, bovine and porcine VAP-B, respectively. VAP-A and VAP-B MSP domains recruit FFAT (two phenylalanines in an acidic tract)-motif-containing proteins to the cytosolic surface of ER membranes (2‑4). FFAT proteins mediate many of the effects of VAPs on regulation of membrane transport, phospholipid biosynthesis, microtubule organization, and the unfolded protein response (2, 3). VAPs also interact with some SNARE and viral proteins (2). A human polymorphism of VAP-B, P56S, is found in three familial motor neuron diseases, notably the amylotrophic lateral sclerosis variant ALS8 (2). It produces a non-functional protein that can dimerize with and inhibit function of normal VAP-B, leading formation of intracellular aggregates and increased ER-stress-induced death of motor neurons (5‑7). It can also promote cleavage and secretion of soluble VAP-B, which can then function as a ligand for EPH receptors (8). A naturally occurring 99 aa isoform of VAP-B that diverges at aa 71 within the MSP domain is termed VAP-C (1, 9). It also appears to be a negative regulator of VAP-A and VAP-B (9). While VAP-B is used by hepatitis C virus (HCV) for its propagation, VAP-C inhibits HCV propagation (9).

  1. Nishimura, Y. et al. (1999) Biochem. Biophys. Res. Commun. 254:21.
  2. Lev, S. et al. (2008) Trends Cell Biol. 18:282.
  3. Peretti, D. et al., 2008, Mol. Biol. Cell 19:3871.
  4. Kaiser, S.E. et al., 2005, Structure 13:1035.
  5. Prosser, D.C. et al. (2008) J. Cell Sci. 121:3052.
  6. Gkogkas, C. et al. (2008) Hum. Mol. Genet. 17:1517.
  7. Suzuki, H. et al. (2009) J. Neurochem. 108:973.
  8. Tsuda, H. et al. (2008) Cell 133:963.
  9. Kukihara, H. et al. (2009) J. Virol. 83:7959.
 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VAPB
Entrez
Uniprot