VAP-B Antibody (699811)

Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VAP‑B was detected in perfusion fixed frozen sections of rat brain (medulla) using Mouse Anti-Rat VAP‑B Monoclonal Antibody (Catalog # MAB7329) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to gigantocellular neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: WB, IHC
• Clone: 699811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VAP-B Antibody (699811) Summary

• Immunogen: E. coli-derived recombinant rat VAP-B
  Ala2-Pro132
  Accession # Q9Z269
• Specificity: Detects rat VAP-B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) VAP-B and approximately 50% cross-reactivity with rhVAP-A is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAPB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAP-B Antibody (699811)

• ALS8
• ALS8VAMP-B/VAMP-C
• VAMP (vesicle-associated membrane protein)-associated protein B and C
• VAMP-associated 33 kDa protein
• VAMP-associated protein B/C
• VAMP-B
• VAMP-C
• VAPB
• VAP-B
• VAP-B/VAP-C
• VAPC
• VAP-C
• vesicle-associated membrane protein-associated protein B/C

Background

Vesicle-associated membrane protein (VAMP)-associated protein B (VAP-B; also VAMP-B) is an ~30 kDa ubiquitously expressed type IV transmembrane protein belonging to the VAP family. It is found in endoplasmic reticulum (ER), Golgi and other membranes as a homodimer or a heterodimer with VAP-A, probably associating through the transmembrane regions. Human VAP-B cDNA encodes a 222 amino acids (aa) cytoplasmic domain and a 21 aa C-terminal membrane anchor. Rat VAP-B shares 89% and 96% aa identity with mouse and human VAP-B, respectively. A soluble form can function as a ligand for EPH receptors. A human polymorphism of VAP-B, P56S, is found in three familial motor neuron diseases, notably the amylotrophic lateral sclerosis variant ALS8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAP-B Antibody (MAB7329)(1)

Publication using MAB7329

• M Petkovic, J Oses-Priet, A Burlingame, LY Jan, YN Jan TMEM16K is an interorganelle regulator of endosomal sorting Nat Commun, 2020-07-03;11(1):3298. 2020-07-03 [PMID: 32620747] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: VAPB
• Uniprot:
  • Q9Z269()