Recombinant Human VAMP-5 Protein

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SDS-Page: VAMP5 Protein [NBC1-22935] - VAMP5, 12.7 kDa (109 aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human VAMP-5 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-72 of Human VAMP-5

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAG IELERCQQQA NEVTEIMRNN FGKVLERGVK LAELQQRSDQ LLDMSSTFNK TTQNLAQKKC WENIRYRIC

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
VAMP5
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
12.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl, 0.5mM EDTA
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human VAMP-5 Protein

  • Myobrevin
  • VAMP5
  • VAMP-5
  • vesicle-associated membrane protein 5 (myobrevin)
  • vesicle-associated membrane protein 5

Background

VAMP5, also known as vesicle-associated membrane protein 5, is a member of the synaptobrevin family and the SNARE superfamily. VAMP5 is the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. This protein may participate in a trafficking events that is associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking. Recombinant Vamp5 protein was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol VAMP5
Entrez