Recombinant Human VAMP-5 His Protein

SDS-Page: Recombinant Human VAMP-5 His Protein [NBC1-22935] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human VAMP-5 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-72 of Human VAMP-5
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAG IELERCQQQA NEVTEIMRNN FGKVLERGVK LAELQQRSDQ LLDMSSTFNK TTQNLAQKKC WENIRYRIC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: VAMP5
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 12.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 5mM DTT, 0.2 M NaCl, 0.5 MM EDTA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human VAMP-5 His Protein

• Myobrevin
• VAMP5
• VAMP-5
• vesicle-associated membrane protein 5 (myobrevin)
• vesicle-associated membrane protein 5

Background

VAMP5, also known as vesicle-associated membrane protein 5, is a member of the synaptobrevin family and the SNARE superfamily. VAMP5 is the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. This protein may participate in a trafficking events that is associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking. Recombinant Vamp5 protein was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: VAMP5