VAMP-5 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

VAMP-5 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human VAMP-5
  Ala2-Cys72
  Accession # O95183
• Specificity: Detects human VAMP-5 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) VAMP-7 is observed, and less than 1% cross-reactivity with rhVAMP-1, rhVAMP-2, and rhVAMP-8 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-5 Antibody [Alexa Fluor® 488]

• Myobrevin
• VAMP5
• VAMP-5
• vesicle-associated membrane protein 5 (myobrevin)
• vesicle-associated membrane protein 5

Background

VAMP-5 (vesicle associated membrane protein 5; also myobrevin) is a member of the synaptobrevin family of proteins. Although its predicted MW is 11 kDa, it runs anomalously at 16 kDa in SDS-Page. VAMP-5 is a type IV transmembrane (TM) protein (i.e.-a type II TM protein whose C-terminus is almost completely transmembrane) that is found in both trans-Golgi and plasma membranes of myotubes (mature skeletal and cardiac muscle cells). While VAMPs are commonly associated with vesicle fusion to t-SNARE in cell membranes, VAMP-5 appears to show no such activity. Human VAMP-5 is 116 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-72) with one vSNARE coiled-coil homology domain, a 21 aa TM segment, and 23 aa C-terminal luminal domain. Over aa 1-72, human VAMP-5 shares 75% aa identity with mouse VAMP-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.