VAMP-2 Antibody (JM11-00)

Western Blot: VAMP-2 Antibody (JM11-00) [NBP2-66774] - Analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY5Y Lane 2: Jurkat

Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody (JM11-00) [NBP2-66774] - Staining VAMP2 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: VAMP-2 Antibody (JM11-00) [NBP2-66774] - Flow cytometric analysis of SH-SY5Y cells with VAMP-2 antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody (JM11-00) [NBP2-66774] - Staining VAMP2 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody (JM11-00) [NBP2-66774] - Staining VAMP2 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

VAMP-2 Antibody (JM11-00) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human VAMP-2 aa 9-42 / 116. (SwissProt: P63027 Human; SwissProt: P63044 Mouse; SwissProt: P63045 Rat)
• Localization: Cell junction, Cell membrane, Cytoplasmic vesicle , Synapse, Synaptosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VAMP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for VAMP-2 Antibody (JM11-00)

• FLJ11460
• SYB2
• SYB2vesicle-associated membrane protein 2
• synaptobrevin 2
• synaptobrevin-2
• VAMP2
• VAMP-2
• VAMP-2vesicle associated membrane protein 2
• vesicle-associated membrane protein 2 (synaptobrevin 2)

Background

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxylterminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons ssociated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAMP2