VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free

Representative section of formalin fixed, paraffin-embedded rat brain stem showing facial motor neurons stained by NBP3-52348 (the BSA Free version of this antibody) using goat anti-chicken IgY secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei.

Western blotting of mock transfected (A) and VAChT/SLC18A3 transfected (B) COS-7 lysates with VAChT/SLC18A3 mouse monoclonal antibody and NBP3-52348 (the BSA Free version of this antibody). The mouse monoclonal antibody and chicken monoclonal antibody were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The chicken monoclonal antibody recognizes exogenous VAChT/SLC18A3 with similar sensitivity as the mouse monoclonal antibody only in VAChT/SLC18A3 transfected COS-7 lysates at ~60 kDa.

• Reactivity: Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: N425/45
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and and buffer exchanged into 1X PBS.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT/SLC18A3 (Uniprot#: Q62666) produced recombinantly in E. Coli
• Specificity: Does not cross-react with VMAT1 or VMAT2
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: SLC18A3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for VAChT/SLC18A3 Antibody (N425/45) - Chimeric - Azide and BSA Free

• MGC12716
• SLC18A3
• solute carrier family 18 (vesicular acetylcholine), member 3
• Solute carrier family 18 member 3
• solute carrier family 18, member 3
• VAChT
• VACHTvesicular acetylcholine transporter

Background

Vesicular Acetylcholine Transporter (VAChT), (approx. 70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC18A3