USP36 Antibody (OTI7G3) - Azide and BSA Free

Western Blot: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP36 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP36.

Immunocytochemistry/Immunofluorescence: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP36.

Immunohistochemistry: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-USP36 mouse monoclonal antibody.

Western Blot: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-USP36 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP36 mouse monoclonal antibody.

Immunohistochemistry: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-USP36 mouse monoclonal antibody.

Immunohistochemistry: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Staining of paraffin-embedded Human liver tissue using anti-USP36 mouse monoclonal antibody.

• Reactivity: Hu, Ca
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: OTI7G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

USP36 Antibody (OTI7G3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse USP36 Antibody (OTI7G3) - Azide and BSA Free (NBP2-03830) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-USP36 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 589-972 of human USP36 (NP_079366) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP36
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500-2000
• Application Notes: Use in KD reported in scientific literature (PMID:34526665).
• Theoretical MW: 122.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for USP36 Antibody (OTI7G3) - Azide and BSA Free

• Deubiquitinating enzyme 36
• DUB1
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ12851
• KIAA1453
• KIAA1453deubiquitinating enzyme 1
• ubiquitin carboxyl-terminal hydrolase 36
• ubiquitin specific peptidase 36
• ubiquitin specific protease 36
• ubiquitin thioesterase 36
• Ubiquitin thiolesterase 36
• Ubiquitin-specific-processing protease 36
• USP36

Background

USP36 belongs to the peptidase C19 family and is a deubiquitinating enzyme. USP36 contains the PEST motif and is polyubiquitinated. USP36 is broadly expressed. There are two named isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP36 Antibody (NBP2-74806)(1)

Publication using NBP2-74806

• Iden M, Tsaih SW, Huang YW et al. Multi-omics mapping of human papillomavirus integration sites illuminates novel cervical cancer target genes Br J Cancer 2021-09-15 [PMID: 34526665] (WB, KD, Human)
  
  Details:
  Citation using the Azide and BSA Free format of this antibody.

Bioinformatics

• Gene Symbol: USP36