USP18 Antibody

Images

 
Western Blot: USP18 Antibody [NB600-893] - Analysis of a band 43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

USP18 Antibody Summary

Immunogen
Synthetic peptide: QPRERPRAWDC, corresponding to amino acids 40 - 49 of Human UBP43.
Specificity
UBP43
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP18
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a band approximately 43 kDa in size corresponding - uBP43 by western blotting in the appropriate cell lysate or extract.
Positive Control
USP18 Lysate (NBL1-17648)

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for USP18 Antibody

  • EC 3.1.2.15
  • EC 3.4.19.-
  • hUBP43
  • ISG15-specific-processing protease
  • ISG43
  • ISG43UBP43
  • ubiquitin specific peptidase 18
  • ubiquitin specific protease 18
  • ubl carboxyl-terminal hydrolase 18
  • ubl thioesterase 18,43 kDa ISG15-specific protease
  • Ubl thiolesterase 18
  • UBP43
  • USP18

Background

UBP43, also known as ISG43 or USP18 is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first bona fide ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP18
Entrez