Recombinant Human Uroplakin III His Protein

SDS-Page: Recombinant Human Uroplakin III His Protein [NBP1-72543] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human Uroplakin III His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 19-207 of Human Uroplakin III
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UPK3A
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 150 mM NaCl
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Uroplakin III His Protein

• MGC119178
• UP3a
• UPIII
• UPIIIA
• UPK3
• UPK3A
• uroplakin 3
• uroplakin 3A
• Uroplakin III
• uroplakin3a
• uroplakin-3a

Background

UPK3A belongs to the uroplakin-3 family. Uroplakins (UP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (UPII, UPIIIa, and UPIIIb) or four times (UPIa and UPIb; both belonging to the 'tetraspanin' family). All have large luminal/extracellular domains, but only UPIIIa and UPIIIb have significant cytoplasmic portions in their C-termini. UPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia Recombinant human UPK3A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Uroplakin III Recombinant Protein (NBP1-72543). (Showing 1 - 1 of 1 FAQ).

1. I have a question about your Uroplakin III protein (#NBP1-72543). How could you measure Uroplakin III activity after purify? cause i need this protein later if activity is good.
   • The lab has informed me that because we haven't measured the activity of Uroplakin III, we regrettably don't have any information or useful advice.

Bioinformatics

• Gene Symbol: UPK3A