UQCRC2 Antibody (JE32-56)

Western Blot: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Western blot analysis of UQCRC2 on different lysates with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Immunocytochemistry analysis of HepG2 cells labeling UQCRC2 with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Flow cytometric analysis of HepG2 cells labeling UQCRC2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33085, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JE32-56
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

UQCRC2 Antibody (JE32-56) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human UQCRC2 aa 285-384 / 453. (Uniprot: P22695)
• Localization: Mitochondrion inner membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UQCRC2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:2000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for UQCRC2 Antibody (JE32-56)

• Complex III subunit 2
• Core protein II
• cytochrome b-c1 complex subunit 2, mitochondrial
• QCR2
• ubiquinol-cytochrome c reductase core protein II
• Ubiquinol-cytochrome-c reductase complex core protein 2
• UQCR2

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex),which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UQCRC2