UPF3A Antibody

Western Blot: UPF3A Antibody [H00065110-B01P] - Analysis of UPF3A expression in transfected 293T cell line by UPF3A polyclonal antibody. Lane 1: UPF3A transfected lysate(48.73 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: UPF3A Antibody [H00065110-B01P] - Analysis of purified antibody to UPF3A on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

UPF3A Antibody Summary

• Immunogen: UPF3A (NP_542418.1, 1 a.a. ~ 443 a.a) full-length human protein.MRSEKEGAGGLRAAVAARGPSGREKLSALEVQFHRDSQQQEAETPPTSSSGCGGGAGKPREEKRTALSKVVIRRLPPGLTKEQLEEQLRPLPAHDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKDPEYKKFLETYCVEEEKTSANPETLLGEMEAKTRELIARRTTPLLEYIKNRKLEKQRIREEKREERRRRELEKKRLREEEKRRRREEERCKKKETDKQKKIAEKEVRIKLLKKPE
• Specificity: Reacts with UPF3 regulator of nonsense transcripts homolog A (yeast).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: UPF3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
• Application Notes: This antibody is reactive against transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UPF3A Antibody

• HUPF3A
• Nonsense mRNA reducing factor 3A
• regulator of nonsense transcripts 3A
• RENT3AUp-frameshift suppressor 3 homolog A
• UPF3 regulator of nonsense transcripts homolog A (yeast)
• UPF3hUpf3

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UPF3A
• Entrez:
  • 65110(Human)
• Uniprot:
  • NP_542418.1(Human)