UPF3A Antibody - Azide and BSA Free

Images

 
Western Blot: UPF3A Antibody [H00065110-B01P] - Analysis of UPF3A expression in transfected 293T cell line by UPF3A polyclonal antibody. Lane 1: UPF3A transfected lysate(48.73 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: UPF3A Antibody [H00065110-B01P] - Analysis of purified antibody to UPF3A on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

UPF3A Antibody - Azide and BSA Free Summary

Immunogen
UPF3A (NP_542418.1, 1 a.a. - 443 a.a.) full-length human protein. MRSEKEGAGGLRAAVAARGPSGREKLSALEVQFHRDSQQQEAETPPTSSSGCGGGAGKPREEKRTALSKVVIRRLPPGLTKEQLEEQLRPLPAHDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKDPEYKKFLETYCVEEEKTSANPETLLGEMEAKTRELIARRTTPLLEYIKNRKLEKQRIREEKREERRRRELEKKRLREEEKRRRREEERCKKKETDKQKKIAEKEVRIKLLKKPEKGEEPTTEKPKERGEEIDTGGGKQESCAPGAVVKARPMEGSLEEPQETSHSGSDKEHRDVERSQEQESEAQRYHVDDGRRHRAHHEPERLSRRSEDEQRWGKGPGQDRGKKGSQDSGAPGEAMERLGRAQRCDDSPAPRKERLANKDRPALQLYDPGARFRARECGGNRRICKAEGSGTGPEKREEAE
Specificity
Reacts with UPF3 regulator of nonsense transcripts homolog A (yeast).
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
UPF3A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
Application Notes
This antibody is reactive against transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UPF3A Antibody - Azide and BSA Free

  • HUPF3A
  • Nonsense mRNA reducing factor 3A
  • regulator of nonsense transcripts 3A
  • RENT3AUp-frameshift suppressor 3 homolog A
  • UPF3 regulator of nonsense transcripts homolog A (yeast)
  • UPF3hUpf3

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol UPF3A
Entrez
Uniprot