UNC5H3/UNC5C Antibody [Unconjugated]

UNC5H3 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human UNC5H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1005) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

UNC5H3/UNC5C Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human UNC5H3
  Ala40-Asp376
  Accession # O95185
• Specificity: Detects human UNC5H3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UNC5C
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC5H3/UNC5C Antibody [Unconjugated]

• netrin receptor UNC5C
• Protein unc-5 homolog 3
• Protein unc-5 homolog C
• unc-5 homolog 3
• unc-5 homolog C (C. elegans)
• UNC5C
• UNC5H3
• UNC5H3unc5 (C.elegans homolog) c

Background

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). UNC5 family proteins are receptors for the Netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are Laminin-related proteins. Netrin family proteins can act as chemoattractants for some axons and as chemorepellents for others. Besides UNC5, Netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven-transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, Netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to Netrin is converted to repulsion by binding of UNC5 to the DCC-Netrin complex. In vivo, the mechanisms of Netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of Netrin (6). Human UNC5H3 cDNA encodes a 931 amino acid (aa) type I membrane protein with a putative 39 aa signal peptide and 337 aa extracellular domain. The extracellular domain of human UNC5H3 shares approximately 98%, 73%, and 66% aa sequence similarity with mouse UNC5H3, rat UNC5H2, and human UNC5H4, respectively.

1. Hong, K. et al. (1999) Cell 97:927.
2. Leonardo, E.D. et al. (1997) Nature 386:833.
3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
4. Corset, V. (2000) Nature 407:747.
5. Merz, D.C. (2001) Genetics 158:1071.
6. Llambi, F. et al. (2001) EMBO J. 20:2715.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UNC5H3/UNC5C Antibody (AF1005)(1)

Publication using AF1005

• Purohit , Anish A, Li , Weiquan, Qu , Chao, Dwyer , Trisha, Shao , Qiangqia, Guan , Kun-Lian, Liu , Guofa Down syndrome cell adhesion molecule (DSCAM) associates with uncoordinated-5C (UNC5C) in netrin-1-mediated growth cone collapse. J Biol Chem, 2012-06-08;287(32):27126-38. 2012-06-08 [PMID: 22685302] (ICC, IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: UNC5C
• Uniprot:
  • O95185()