UHRF1 Antibody (2A8C7) - BSA Free

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Western Blot: UHRF1 Antibody (2A8C7) [NBP2-61830] - Analysis using UHRF1 mAb against HEK293 (1) and UHRF1 (AA: 616-755)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: UHRF1 Antibody (2A8C7) [NBP2-61830] - Analysis of paraffin-embedded esophageal cancer tissues using UHRF1 mouse mAb with DAB staining.
Western Blot: UHRF1 Antibody (2A8C7) [NBP2-61830] - Analysis using UHRF1 mAb against human UHRF1 (AA: 616-755) recombinant protein. (Expected MW is 41.8 kDa)
Western Blot: UHRF1 Antibody (2A8C7) [NBP2-61830] - Analysis using UHRF1 mouse mAb against MCF-7 (1), HCT116 (2), HL-60 (3), Hela (4), and HEK293 (5) cell lysate.
ELISA: UHRF1 Antibody (2A8C7) [NBP2-61830] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2A8C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

UHRF1 Antibody (2A8C7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
UHRF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
89.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for UHRF1 Antibody (2A8C7) - BSA Free

  • E3 ubiquitin-protein ligase UHRF1
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ21925
  • hNp95
  • hUHRF1
  • HuNp95
  • ICBP90
  • ICBP90NP95
  • Inverted CCAAT box-binding protein of 90 kDa
  • NP95
  • Nuclear protein 95
  • Nuclear zinc finger protein Np95
  • RING finger protein 106
  • RNF106
  • RNF106MGC138707
  • Transcription factor ICBP90
  • Ubiquitin-like PHD and RING finger domain-containing protein 1
  • ubiquitin-like with PHD and ring finger domains 1
  • ubiquitin-like, containing PHD and RING finger domains, 1
  • Ubiquitin-like-containing PHD and RING finger domains protein 1
  • UHRF1

Background

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

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Bioinformatics

Gene Symbol UHRF1