UGRP1/SCGB3A2 Antibody [Unconjugated]

Disruption of Jagged-driven Notch signaling has no detectable impact on PNEC and NEB cell fate specification or maintenance.(A) qPCR analysis of Ascl1 in E18.5 controls, single and double Jag1cnull; Jag2cnull mutants showing no significant difference in expression. Graph represents mean ± SEM (n = 4 Jag1 control, n = 3 Jag1cnull; n = 4 Jag2 control; n = 4 Jag2cnull; n = 4 Jag1/Jag2 control; n = 4 Jag1cnull; Jag2cnull). Student’s t-test was used to analyze data. Side panels: representative Cgrp immunofluorescence (IF) in controls and mutants (B) IF of secretory (Scgb3a2), NEB-associated SSEA1 (CC) and Cgrp (PNEC/NEB) in control and Jag mutants. (C, D) Preserved NEB microenvironment in E18.5 Jag1cnull; Jag2cnull double mutants: IF showing NEBs (Cgrp) and NEB-associated CCs (SSEA1, N1ICD, CC10 low) in double null mutants similar to controls (Ex. inset in D). (E) qPCR analysis of Upk3a in E18.5 control and double Jag1cnull; Jag2cnull mutants showing nearly abolished Upk3a expression in mutants (n = 3 in each group). Graph: mean ± SEM; ***p<0.0005 (F) ISH of Upk3a (left panels) and double labeled with Ascl1 (immunohistochemistry, right panels): Upk3a expression predominantly in NEB-associated CCs with rare signals scattered in CC elsewhere. In double Jag1cnull; Jag2cnull mutants CCs are abolished but in the NEB microenvironment and Upk3a becomes restricted to those surrounding NEBs. Scale bar in A-D, F = 40 μm. Scale bar in F inset = 20 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31631837), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

UGRP1/SCGB3A2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse UGRP1/SCGB3A2 isoform C
  Leu22-Leu139
  Accession # Q920H1
• Specificity: Detects mouse UGRP1/SCGB3A2 isoforms C and A in direct ELISAs and Western blots. It is also expected to recognize isoform B in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human UGRP1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCGB3A2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UGRP1/SCGB3A2 Antibody [Unconjugated]

• LU103
• Pneumo secretory protein 1
• PnSP-1
• PNSP1secretoglobin family 3A member 2
• SCGB3A2
• secretoglobin, family 3A, member 2
• UGRP1
• UGRP1pnSP-1
• Uteroglobin-related protein 1

Background

UGRP-1, also known as secretoglobin family 3A member 2 (SCGB3A2) and HIN-2, is a secreted homodimeric protein that is highly expressed in epithelial cells of the airway. By alternative splicing events, three isoforms (A, B, C) that differ in their C-terminal regions, exist. UGRP-1 has been shown to bind MARCO (macrophage scavenger receptor with collagenous structure), which is expressed by alveolar macrophages. UGRP1 has been suggested to play a role in lung inflammation. Mature mouse UGRP1 isoform A shares 86% and 81% amino acid sequence identity with rat and human mature UGRP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UGRP1/SCGB3A2 Antibody (AF3465)(11)

Publications using AF3465

• Shen, H;Chen, W;Liu, Y;Castaldi, A;Castillo, J;Horie, M;Flodby, P;Sundar, S;Li, C;Ji, Y;Minoo, P;Marconett, CN;Zhou, B;Borok, Z; GRAMD2 + alveolar type I cell plasticity facilitates cell state transitions in organoid culture bioRxiv : the preprint server for biology 2023-10-18 [PMID: 37905051] (IHC, Mouse)
• Beppu, AK;Zhao, J;Yao, C;Carraro, G;Israely, E;Coelho, AL;Drake, K;Hogaboam, CM;Parks, WC;Kolls, JK;Stripp, BR; Epithelial plasticity and innate immune activation promote lung tissue remodeling following respiratory viral infection Nature communications 2023-09-19 [PMID: 37726288] (IHC, Mouse)
• Aaron I. Weiner, Gan Zhao, Hanna M. Zayas, Nicolas P. Holcomb, Stephanie Adams-Tzivelekidis, Joanna Wong et al. delta Np63 drives dysplastic alveolar remodeling and restricts epithelial plasticity upon severe lung injury Cell Reports 2022-12-13 [PMID: 36516758]
• Y Chen, R Toth, S Chocarro, D Weichenhan, J Hey, P Lutsik, S Sawall, GT Stathopoul, C Plass, R Sotillo Club cells employ regeneration mechanisms during lung tumorigenesis Nature Communications, 2022-08-05;13(1):4557. 2022-08-05 [PMID: 35931677] (IHC, Mouse)
• Reiko Kurotani, Akira Kurumazuka, Satoshi Sakahara, Kei Takakura, Yutaro Yokoyama, Lei Xu et al. Development of Aging-Related Emphysematous and Lymphoma-Like Lesions is Enhanced by the Lack of Secretoglobin 3A2 in Mouse Lungs International Journal of Chronic Obstructive Pulmonary Disease 2022-05-26 [PMID: 35651829]
• Evelien Eenjes, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Gabriela G Edel, Floor Benthem, Lisette de Kreij-de Bruin et al. SOX21 modulates SOX2-initiated differentiation of epithelial cells in the extrapulmonary airways eLife 2021-07-21 [PMID: 34286693]
• JJ Kathiriya, AN Brumwell, JR Jackson, X Tang, HA Chapman Distinct Airway Epithelial Stem Cells Hide among Club Cells but Mobilize to Promote Alveolar Regeneration Cell Stem Cell, 2020-01-23;0(0):. 1/23/2020 [PMID: 31978363]
• Maria R Stupnikov, Ying Yang, Munemasa Mori, Jining Lu, Wellington V Cardoso Jagged and Delta-like ligands control distinct events during airway progenitor cell differentiation eLife 2019-10-21 [PMID: 31631837]
• Snahlata Singh, Emily Elenio, Nicolae A. Leu, Rose‐Anne Romano, Andrew E. Vaughan, Jennifer DeRiso et al. A new Elf5CreERT2-GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues FEBS Letters 2019-05-01 [PMID: 31002388]
• Yuki Miyano, Shigeyuki Tahara, Ichiro Sakata, Takafumi Sakai, Hiroyuki Abe, Shioko Kimura et al. Regulation of LH/FSH expression by secretoglobin 3A2 in the mouse pituitary gland Cell and Tissue Research 2014-04-01 [PMID: 24514953] (Immunohistochemistry, Mouse)
• Thomas M. Raffay, Morgan L. Locy, Cynthia L. Hill, Nik S. Jindal, Lynette K. Rogers, Stephen E. Welty et al. Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1 BioMed Research International 2013-09-26 [PMID: 24187664]

Bioinformatics

• Gene Symbol: SCGB3A2
• Uniprot:
  • Q920H1()