UFL1 Antibody

Western Blot: UFL1 Antibody [NBP1-79039] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ULF1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ULF1 was also immunoprecipitated by rabbit anti-ULF1 antibody NBP1-79038 which recognizes an upstream epitope.

• Reactivity: Hu, Mu, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

UFL1 Antibody Summary

• Description: Novus Biologicals Rabbit UFL1 Antibody (NBP1-79039) is a polyclonal antibody validated for use in WB and IP. Anti-UFL1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 744 and 794 of human E3 UFM1-Protein Ligase 1 using the numbering given in entry NP_056138.1 (GeneID 23376).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UFL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UFL1 Antibody

• E3 UFM1-protein ligase 1
• EC 6.3.2.-
• KIAA0776
• LZAP-binding protein
• NLBPnovel LZAP-binding protein
• RP3-393D12.1
• UFL1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UFL1 Antibody (NBP1-79039)(3)

Publications using NBP1-79039

• Schmidt H, Sorensen G, Lanahan M et al. UFMylation promotes orthoflavivirus infectious particle production Journal of Virology 2025-07-01 [PMID: 40459261]
• Haijiao Jin, Yuanting Yang, Xuying Zhu, Yin Zhou, Yao Xu, Jialin Li, Chaojun Qi, Xinghua Shao, Jingkui Wu, Shan Wu, Hong Cai, Leyi Gu, Shan Mou, Zhaohui Ni, Shu Li, Qisheng Lin DDRGK1-mediated ER-phagy attenuates acute kidney injury through ER-stress and apoptosis Cell Death & Disease 2024-01-17 [PMID: 38233375]
• Wang K, Chen S, Wu Y et al. The ufmylation modification of ribosomal protein L10 in the development of pancreatic adenocarcinoma Cell death & disease 2023-06-07 [PMID: 37280198] (WB, Mouse)

Bioinformatics

• Gene Symbol: UFL1
• Uniprot:
  • NP_056138.1(Human)