UEV2 Antibody

Product Discontinued

UEV2 Antibody Summary

• Immunogen: Peptide with sequence AVSTGVKVPRNF-C corresponding to N-Terminus according to NP_003341.1, NP_954673.1.
• Specificity: This antibody is also expected to recognize the readthrough transcript product KUA-UEV (NP_954673.1, GeneID 387522). Also cross-reactivity is expected with UEV2V1 (GeneID 7335).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBE2V2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 1 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in human brain lysate.
• Control:

  UEV2 Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UEV2 Antibody

• 1 alpha
• 25-dihydroxyvitamin D3-inducible
• DDVIT1
• DDVit-1
• EDAF-1
• EDPF1
• EDPF-1DDVit 1
• enterocyte differentiation promoting factor
• Enterocyte differentiation-associated factor 1
• enterocyte differentiation-associated factor EDAF-1
• Enterocyte differentiation-promoting factor 1
• methyl methanesulfonate sensitive 2, S. cerevisiae, homolog of
• MMS2
• MMS2MMS2 homolog
• UBE2V2
• ubiquitin-conjugating enzyme E2 variant 2
• Uev2
• UEV-2
• Vitamin D3-inducible protein

Background

The differentiation and activation of monocytes (MO) and monocytic cells is modulated by Vitamin D3. Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3. This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2P and human CROC-1, which defines a structurally and functionally conserved UBC-like protein family. This protein was called UEV2. The yeast UEV protein is required together with UBC13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast. These proteins also play a central role in the assembly of K63-linked polyubiquitin chains UEV/UBC complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the UEV2 heterodimer is necessary for DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UEV2 Antibody (NB100-1026)(1)

Publication using NB100-1026

• Fritsche J, Rehli M, Krause SW et al. Molecular cloning of a 1alpha,25-dihydroxyvitamin D3-inducible transcript (DDVit 1) in human blood monocytes. Biochem Biophys Res Commun 1997-06-18 [PMID: 9199207]

Bioinformatics

• Gene Symbol: UBE2V2
• Entrez:
  • 7336(Human)
  • 70620(Mouse)
• Uniprot:
  • NP_003341(Human)