Recombinant Human UCH-L5/UCH37 His Protein

SDS-Page: Recombinant Human UCH-L5/UCH37 Protein [NBP1-72315] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.5 mg/ml

Recombinant Human UCH-L5/UCH37 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-329 of Human UCH-L5/UCH37
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK

• Details of Functionality: Specific activity > 500 pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UCHL5
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 39.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 5mM DTT, 30% glycerol, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human UCH-L5/UCH37 His Protein

• CGI-70
• EC 3.4.19.12
• INO80 complex subunit R
• INO80R
• ubiquitin carboxyl-terminal esterase L5
• ubiquitin carboxyl-terminal hydrolase isozyme L5
• ubiquitin carboxyl-terminal hydrolase L5
• Ubiquitin C-terminal hydrolase UCH37
• Ubiquitin thioesterase L5
• UCH37
• UCH37CGI-70
• UCHL5
• UCH-L5

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human UCHL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UCHL5