UCH-L3 Antibody

Images

 
Western Blot: UCH-L3 Antibody [NBP1-49711] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity ...read more
Immunohistochemistry: UCH-L3 Antibody [NBP1-49711] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- UCH-L3 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

UCH-L3 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 174 and 224 of human Ubiquitin Carboxyl-Terminal Esterase L3 using the numbering given in entry NP_005993.1 (GeneID 7347).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCHL3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UCH-L3 Antibody

  • EC 3.4.19.12
  • ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L3
  • Ubiquitin Thioesterase L3
  • ubiquitin thiolesterase
  • UCHL3
  • UCH-L3

Background

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Four distinct UCH activities from bovine thymus have been identified. All 4 are thiol proteases and have high-affinity binding sites for ubiquitin. UCHL3 protein is 54% identical to that of UCHL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UCHL3
Entrez