UBR4 Antibody

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-45235] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UBR4 Antibody Summary

• Description: Novus Biologicals Goat UBR4 Antibody (NBP1-45235) is a polyclonal antibody validated for use in IHC and ELISA. Anti-UBR4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NTTRATSREEKNLQ corresponding to C-Terminus according to NP_065816.2.
• Epitope: C-Terminus
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the calculated size of 574 kDa band according to NP_065816.2. IHC-P: Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBR4 Antibody

• 600 kDa retinoblastoma protein-associated factor
• EC 6.3.2
• EC 6.3.2.-
• KIAA0462E3 ubiquitin-protein ligase UBR4
• KIAA1307zinc finger, UBR1 type 1
• N-recognin-4
• p600
• RBAF600FLJ41863
• Retinoblastoma-associated factor of 600 kDa
• ubiquitin protein ligase E3 component n-recognin 4
• Zinc finger UBR1-type protein 1
• ZUBR1retinoblastoma-associated factor 600-like protein

Background

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBR4 Antibody (NBP1-45235)(1)

Publication using NBP1-45235

• Lennerz V, Fatho M, Gentilini C et al. The response of autologous T cells to a human melanoma is dominated by mutated neoantigens. Proc Natl Acad Sci U S A 2005-11-01 [PMID: 16247014]

Bioinformatics

• Gene Symbol: UBR4
• Entrez:
  • 23352(Human)
  • 69116(Mouse)
  • 313658(Rat)
• Uniprot:
  • NP_065816.2(Human)