UBR4 Antibody (2D12) - Azide and BSA Free

Western Blot: UBR4 Antibody (2D12) [H00023352-M01] - RBAF600 expression in K-562 ( Cat # L009V1 ).

Immunocytochemistry/Immunofluorescence: UBR4 Antibody (2D12) [H00023352-M01] - Analysis of monoclonal antibody to RBAF600 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: UBR4 Antibody (2D12) [H00023352-M01] - Detection limit for recombinant GST tagged RBAF600 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UBR4 Antibody (2D12) - Azide and BSA Free Summary

• Immunogen: RBAF600 (NP_065816, 94 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNQLQSVAAACKVLIEFSLLRLENPDEACAVSQKHLILLIKGLCTGCSRLDRTEIITFTAMMKSAKLPQTVKTLSDVEDQKELASPVSPELRQKEVQ
• Specificity: RBAF600 - retinoblastoma-associated factor 600
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBR4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UBR4 Antibody (2D12) - Azide and BSA Free

• 600 kDa retinoblastoma protein-associated factor
• EC 6.3.2
• EC 6.3.2.-
• KIAA0462E3 ubiquitin-protein ligase UBR4
• KIAA1307zinc finger, UBR1 type 1
• N-recognin-4
• p600
• RBAF600FLJ41863
• Retinoblastoma-associated factor of 600 kDa
• ubiquitin protein ligase E3 component n-recognin 4
• Zinc finger UBR1-type protein 1
• ZUBR1retinoblastoma-associated factor 600-like protein

Background

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBR4
• Entrez:
  • 23352(Human)
• Uniprot:
  • NP_065816(Human)