Recombinant Human Ubiquitin C Protein

SDS-Page: ubiquitin C Protein [NBC1-18439] - Ubiquitin 8.6 kDa (76 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Ubiquitin C Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-76 of Human Ubiquitin C
  
  

  Source: E.coli

  Amino Acid Sequence: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBC
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 8.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM HEPES buffer (pH 7.4) containing 150mM NaCl, 1mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Ubiquitin C Protein

• HMG20
• PolyUbiquitin
• polyubiquitin-B
• Polyubiquitin-C
• UBC
• Ubiquitin-40S ribosomal protein S27a
• ubiquitin-60S ribosomal protein L40

Background

Ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity. Ubiquitin(76amino acid) was overexpressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBC