UBE2S Antibody (OTI1B5) - Azide and BSA Free

Western Blot: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S.

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by UBE2S antibody.

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of Hela cells, using UBE2S antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of Jurkat cells, using UBE2S antibody,(Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI1B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

UBE2S Antibody (OTI1B5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human UBE2S(NP_055316) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2S
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot 1:2000
• Theoretical MW: 23.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for UBE2S Antibody (OTI1B5) - Azide and BSA Free

• E2-24K
• E2-EPF
• E2-EPF5
• E2-EPF52
• E2-EPFEC 6.3.2.19
• E2EPFEPF5
• EPF5
• UBE2S
• Ubiquitin carrier protein S
• ubiquitin-conjugating enzyme E2 S
• ubiquitin-conjugating enzyme E2-24 kD
• Ubiquitin-conjugating enzyme E2-24 kDa
• Ubiquitin-conjugating enzyme E2-EPF5
• ubiquitin-conjugating enzyme E2S
• Ubiquitin-protein ligase S

Background

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2S