UBE2R1/CDC34 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.

Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

UbcH3/Cdc34 Antibody [NBP1-32153] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBE2R1/CDC34 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UBE2R1/CDC34 Antibody - BSA Free (NBP1-32153) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE2R1/CDC34. The exact sequence is proprietary.
• Predicted Species: Rat (98%), Xenopus (90%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC34
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (90%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBE2R1/CDC34 Antibody - BSA Free

• Cdc34
• cell division cycle 34 homolog (S. cerevisiae)
• cell division cycle 34
• E2-CDC34
• EC 6.3.2.19
• UBC3
• UBE2R1
• UBE2R1UBCH3
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 R1
• Ubiquitin-conjugating enzyme E2-32 kDa complementing
• Ubiquitin-conjugating enzyme E2-CDC34
• Ubiquitin-protein ligase R1

Background

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CDC34