UBE2R1/CDC34 Antibody (90.12)

Western Blot: Cdc34 Antibody (90.12) [NB500-110] - Detection of human CDC34 protein in DU145 whole cell lysate.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 90.12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

UBE2R1/CDC34 Antibody (90.12) Summary

• Immunogen: Full-length human Cdc34 expressed in E. coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC34
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5 ug/ml
  • Western Blot 1-5 ug/ml
• Control:

  293 Whole Cell Lysate
  UBE2R1/CDC34 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for UBE2R1/CDC34 Antibody (90.12)

• Cdc34
• cell division cycle 34 homolog (S. cerevisiae)
• cell division cycle 34
• E2-CDC34
• EC 6.3.2.19
• UBC3
• UBE2R1
• UBE2R1UBCH3
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 R1
• Ubiquitin-conjugating enzyme E2-32 kDa complementing
• Ubiquitin-conjugating enzyme E2-CDC34
• Ubiquitin-protein ligase R1

Background

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2R1/CDC34 Antibody (NB500-110)(1)

Publication using NB500-110

• Fernandez-Sanchez ME, Sechet E, Margottin-Goguet F et al. The Human COP9 Signalosome Protects Ubiquitin-conjugating Enzyme 3 (UBC3/Cdc34) from {beta}-Transducin Repeat-containing Protein ({beta}TrCP)-mediated Degradation. J Biol Chem. 2010-04-08 [PMID: 20378537]

Bioinformatics

• Gene Symbol: CDC34
• Entrez:
  • 997(Human)
• Uniprot:
  • P49427(Human)