Recombinant Human UBE2M/Ubc12 Protein

• Reactivity: Hu
• Applications: PAGE
• Format: Carrier-Free

Recombinant Human UBE2M/Ubc12 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 183 amino acids corresponding to UBE2M/Ubc12 Source: E. coli

  Uniprot ID: P61081

  Amino Acid Sequence: MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2M
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of UBE2M/Ubc12 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 20.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 6.5, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
• Preservative: 5% Trehalose
• Purity: >95%, by SDS-PAGE and HPLC

Alternate Names for Recombinant Human UBE2M/Ubc12 Protein

• EC 6.3.2
• EC 6.3.2.-
• hUbc12
• NEDD8 carrier protein
• NEDD8 protein ligase
• UBC12
• UBC12NEDD8-conjugating enzyme Ubc12
• UbcH12
• UBC-RS2
• UBE2M
• ubiquitin carrier protein M
• Ubiquitin-conjugating enzyme E2 M
• ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
• ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• ubiquitin-protein ligase M
• yeast UBC12 homolog

Background

Human Ubquitin Conjugating Enzyme E2 M (UBE2M)/UBC12 is is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APPBP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF (beta-TRCP) complex which is important as the E3-ligase in the ubiquitinylation of I-kappaB-alpha. NEDDylation of Cul-1 is essential for conjugation and processing of NF-kappaB p105 by SCF (beta-TRCP) following phosphorylation of the complex. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2M
• Uniprot:
  • P61081(Human)