UBE2M/Ubc12 Overexpression Lysate (Native)

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Western Blot: UBE2M Overexpression Lysate (Adult Normal) [NBL1-17540] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2M.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

UBE2M/Ubc12 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208946 and based on accession number NM_003969. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (UBE2M), mRNA.
Gene
UBE2M

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for UBE2M/Ubc12

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UBE2M/Ubc12 Overexpression Lysate (Native)

  • EC 6.3.2
  • EC 6.3.2.-
  • hUbc12
  • NEDD8 carrier protein
  • NEDD8 protein ligase
  • UBC12
  • UBC12NEDD8-conjugating enzyme Ubc12
  • UbcH12
  • UBC-RS2
  • UBE2M
  • ubiquitin carrier protein M
  • Ubiquitin-conjugating enzyme E2 M
  • ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
  • ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
  • ubiquitin-protein ligase M
  • yeast UBC12 homolog

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol UBE2M
Entrez