UbcH7/UBE2L3 Antibody (SR1268) - BSA Free

Western Blot: UbcH7/UBE2L3 Antibody (SR1268) [NBP3-22542] - Western blot analysis using NBP3-22542 in Jurkat cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SR1268
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UbcH7/UBE2L3 Antibody (SR1268) - BSA Free Summary

• Description: Novus Biologicals Rabbit UbcH7/UBE2L3 Antibody (SR1268) - BSA Free (NBP3-22542) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human UbcH7/UBE2L3 (Uniprot #: P68036)
• Specificity: Detects endogenous levels of total UbcH7/UBE2L3
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBE2L3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for UbcH7/UBE2L3 Antibody (SR1268) - BSA Free

• E2-F1
• EC 6.3.2.19
• L-UBC
• UBCE7
• UbcH7
• UBCH7UbcM4
• UbcM4
• UBE2L3
• Ubiquitin carrier protein L3
• ubiquitin-conjugating enzyme E2 L3
• Ubiquitin-conjugating enzyme E2-F1
• ubiquitin-conjugating enzyme E2L 3
• ubiquitin-conjugating enzyme UBCH7
• Ubiquitin-protein ligase L3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2L3