UbcH6/UBE2E1 Antibody [DyLight 488]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

UbcH6/UBE2E1 Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human UBCH6 protein (between residues 1-50) [UniProt P51965]
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:600
  • Immunohistochemistry-Paraffin 1:600
Application Notes
This UBCH6 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus and plasma membrane of mouse intestine tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for UbcH6/UBE2E1 Antibody [DyLight 488]

  • EC 6.3.2.19
  • UbcH6
  • UBE2E1
  • Ubiquitin carrier protein E1
  • ubiquitin-conjugating enzyme E2 E1
  • ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5)
  • ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase E1

Background

Ubiquitination is a cellular process by which the cell identifies abnormal or short-lived proteins for degradation. Three classes of enzymes are involved in this process, ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH6 (UBE2E1 or ubiquitin-conjugating enzyme E2E 1) belongs to the E2 class of enzymes. ISG15, an ubiquitin like protein has been shown to covalently modify UBCH6 as well as forming a thoiester intermediate with ubiquitin. ISG15-modified UBCH6 formed a ubiquitin thioester while the unmodified UBCH6 did not which suggests that ISG156 conjugation suppresses UBCH6 E2 enzyme activity. (PMID: 16428300) In Spinalcerebellar Ataxia-1 (SCA1), research has shown that UBCH6 ubiquinates Ataxin-1. The overexpression of UBCH6 also reduced the transcriptional repression activity of ataxin-1. It addition it was found that overexpression of UbcH6 reduced the formation of Ataxin-1 aggregated. By modulating the degradation of ataxin-1, UbcH6 can regulate the transcriptional repression activity of ataxin-1 (PMID: 18519031).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional UbcH6/UBE2E1 Products

Bioinformatics Tool for UbcH6/UBE2E1 Antibody (NBP1-78983G)

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Bioinformatics

Gene Symbol UBE2E1
Entrez