UbcH6/UBE2E1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: UbcH6/UBE2E1 Antibody [NBP1-78983] - UBCH6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: UbcH6/UBE2E1 Antibody [NBP1-78983] - Analysis of UBCH6 in mouse intestine using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.02 mg/ml

UbcH6/UBE2E1 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human UBCH6 protein (between residues 1-50) [UniProt P51965]
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:600
  • Immunohistochemistry-Paraffin 1:600
Application Notes
This UBCH6 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus and plasma membrane of mouse intestine tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot.
Control
UbcH6/UBE2E1 Knockout 293T Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.02 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UbcH6/UBE2E1 Antibody

  • EC 6.3.2.19
  • UbcH6
  • UBE2E1
  • Ubiquitin carrier protein E1
  • ubiquitin-conjugating enzyme E2 E1
  • ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5)
  • ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase E1

Background

Ubiquitination is a cellular process by which the cell identifies abnormal or short-lived proteins for degradation. Three classes of enzymes are involved in this process, ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH6 (UBE2E1 or ubiquitin-conjugating enzyme E2E 1) belongs to the E2 class of enzymes. ISG15, an ubiquitin like protein has been shown to covalently modify UBCH6 as well as forming a thoiester intermediate with ubiquitin. ISG15-modified UBCH6 formed a ubiquitin thioester while the unmodified UBCH6 did not which suggests that ISG156 conjugation suppresses UBCH6 E2 enzyme activity. (PMID: 16428300) In Spinalcerebellar Ataxia-1 (SCA1), research has shown that UBCH6 ubiquinates Ataxin-1. The overexpression of UBCH6 also reduced the transcriptional repression activity of ataxin-1. It addition it was found that overexpression of UbcH6 reduced the formation of Ataxin-1 aggregated. By modulating the degradation of ataxin-1, UbcH6 can regulate the transcriptional repression activity of ataxin-1 (PMID: 18519031).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for UbcH6/UBE2E1 Antibody (NBP1-78983) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78983AF350
Alexa Fluor 405 NBP1-78983AF405
Alexa Fluor 488 NBP1-78983AF488
Alexa Fluor 532 NBP1-78983AF532
Alexa Fluor 594 NBP1-78983AF594
Alexa Fluor 647 NBP1-78983AF647
Alexa Fluor 700 NBP1-78983AF700
Alexa Fluor 750 NBP1-78983AF750
Biotin NBP1-78983B
DyLight 350 NBP1-78983UV
DyLight 405 NBP1-78983V
DyLight 488 NBP1-78983G
DyLight 550 NBP1-78983R
DyLight 594 NBP1-78983DL594
DyLight 650 NBP1-78983C
DyLight 680 NBP1-78983FR
DyLight 755 NBP1-78983IR
FITC NBP1-78983F
HRP NBP1-78983H
Janelia Fluor 549 NBP1-78983JF549
Janelia Fluor 646 NBP1-78983JF646

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Bioinformatics

Gene Symbol UBE2E1
Entrez
Uniprot