UbcH6/UBE2E1 Antibody

Immunocytochemistry/Immunofluorescence: UbcH6/UBE2E1 Antibody [NBP1-78983] - UBCH6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: UbcH6/UBE2E1 Antibody [NBP1-78983] - Analysis of UBCH6 in mouse intestine using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC, IHC-P, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.02 mg/ml

UbcH6/UBE2E1 Antibody Summary

• Immunogen: A synthetic peptide made to an N-terminal portion of the human UBCH6 protein (between residues 1-50) [UniProt P51965]
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2E1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:600
  • Immunohistochemistry-Paraffin 1:600
• Application Notes: This UBCH6 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus and plasma membrane of mouse intestine tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot.
• Control:

  UbcH6/UBE2E1 Knockout 293T Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.02 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UbcH6/UBE2E1 Antibody

• EC 6.3.2.19
• UbcH6
• UBE2E1
• Ubiquitin carrier protein E1
• ubiquitin-conjugating enzyme E2 E1
• ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5)
• ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase E1

Background

Ubiquitination is a cellular process by which the cell identifies abnormal or short-lived proteins for degradation. Three classes of enzymes are involved in this process, ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH6 (UBE2E1 or ubiquitin-conjugating enzyme E2E 1) belongs to the E2 class of enzymes. ISG15, an ubiquitin like protein has been shown to covalently modify UBCH6 as well as forming a thoiester intermediate with ubiquitin. ISG15-modified UBCH6 formed a ubiquitin thioester while the unmodified UBCH6 did not which suggests that ISG156 conjugation suppresses UBCH6 E2 enzyme activity. (PMID: 16428300) In Spinalcerebellar Ataxia-1 (SCA1), research has shown that UBCH6 ubiquinates Ataxin-1. The overexpression of UBCH6 also reduced the transcriptional repression activity of ataxin-1. It addition it was found that overexpression of UbcH6 reduced the formation of Ataxin-1 aggregated. By modulating the degradation of ataxin-1, UbcH6 can regulate the transcriptional repression activity of ataxin-1 (PMID: 18519031).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2E1
• Entrez:
  • 7324(Human)
• Uniprot:
  • P51965(Human)