UBE2E3 Antibody (OTI7E8)

Images

 
Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.
Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2E3 antibody, and then ...read more
Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human colon tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human Kidney tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
OTI7E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UBE2E3 Antibody (OTI7E8) Summary

Immunogen
Full length human recombinant protein of human UBE2E3 (NP_006348) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
UBE2E3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-03819 in the following applications:

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for UBE2E3 Antibody (OTI7E8)

  • E2-23K
  • EC 6.3.2.19
  • UBCE4
  • UbcH9
  • UBCH9ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5)
  • UbcM2
  • UBE2E3
  • Ubiquitin carrier protein E3
  • ubiquitin-conjugating enzyme E2 E3
  • Ubiquitin-conjugating enzyme E2-23 kDa
  • ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase E3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for UBE2E3 Antibody (NBP2-03819) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-03819:
Filter by Applications
ICC/IF
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence UBE2E3 NBP2-03819
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5
reviewed by:
Anonymous
ICC/IF Mouse 10/11/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedPC3 prostate cancer cell line
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for UBE2E3 Antibody (NBP2-03819) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74745AF350
Alexa Fluor 405 NBP2-74745AF405
Alexa Fluor 488 NBP2-74745AF488
Alexa Fluor 532 NBP2-74745AF532
Alexa Fluor 594 NBP2-74745AF594
Alexa Fluor 647 NBP2-74745AF647
Alexa Fluor 700 NBP2-74745AF700
Alexa Fluor 750 NBP2-74745AF750
Allophycocyanin NBP2-74745APC
Biotin NBP2-74745B
DyLight 350 NBP2-74745UV
DyLight 405 NBP2-74745V
DyLight 488 NBP2-74745G
DyLight 550 NBP2-74745R
DyLight 594 NBP2-74745DL594
DyLight 650 NBP2-74745C
DyLight 680 NBP2-74745FR
DyLight 755 NBP2-74745IR
FITC NBP2-74745F
HRP NBP2-74745H
Janelia Fluor 549 NBP2-74745JF549
Janelia Fluor 646 NBP2-74745JF646
PE NBP2-74745PE
PerCP NBP2-74745PCP

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/11/2016
Application: ICC/IF
Species: Mouse

Bioinformatics

Gene Symbol UBE2E3
Entrez