UbcH5a/UBE2D1 Antibody - BSA Free

Western Blot: UbcH5a/UBE2D1 Antibody [NBP2-93678] - Analysis of extracts of various cell lines, using UbcH5a/UBE2D1 .

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP2-93678] - Paraffin-embedded rat spleen using UbcH5a/UBE2D1 .

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP2-93678] - Paraffin-embedded mouse spleen using UbcH5a/UBE2D1 .

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP2-93678] - Paraffin-embedded rat brain using UbcH5a/UBE2D1 .

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UbcH5a/UBE2D1 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D1 (NP_003329.1). MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2D1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for UbcH5a/UBE2D1 Antibody - BSA Free

• E2(17)KB1
• Stimulator of Fe transportUBC4/5 homolog
• UBC4/5
• UBC5A
• UbcH5
• UbcH5a
• UBCH5AEC 6.3.2.19
• UBCH5SFT
• UBE2D1
• Ubiquitin carrier protein D1
• ubiquitin-conjugating enzyme E2 D1
• Ubiquitin-conjugating enzyme E2(17)KB 1
• Ubiquitin-conjugating enzyme E2-17 kDa 1
• ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D1

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2D1