UbcH10/UBE2C Antibody

Images

 
Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - Expression in transfected 293T cell line by UBE2C polyclonal antibody. A: Non-transfected lysate. B: UBE2C transfected lysate.
Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody [NBP2-20782] - A549 cells. Green: UBE2C protein stained by UBE2C antibody [N1C3]. Blue: Hoechst 33342 staining.
Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - A:NIH-3T3.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UbcH10/UBE2C Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2C. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UbcH10/UBE2C Antibody

  • cyclin-selective ubiquitin carrier protein
  • dJ447F3.2
  • EC 6.3.2.19
  • UbcH10
  • UBCH10mitotic-specific ubiquitin-conjugating enzyme
  • UBE2C
  • Ubiquitin carrier protein C
  • ubiquitin carrier protein E2-C
  • ubiquitin-conjugating enzyme E2 C
  • ubiquitin-conjugating enzyme E2C
  • Ubiquitin-protein ligase C

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UBE2C
Entrez
Uniprot