UbcH10/UBE2C Antibody - BSA Free

Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UBE2C antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody [NBP2-20782] - A549 cells. Green: UBE2C protein stained by UBE2C antibody [N1C3]. Blue: Hoechst 33342 staining.

Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - A:NIH-3T3.

Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - Expression in transfected 293T cell line by UBE2C polyclonal antibody. A: Non-transfected lysate. B: UBE2C transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UbcH10/UBE2C Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human UbcH10/UBE2C. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UbcH10/UBE2C Antibody - BSA Free

• cyclin-selective ubiquitin carrier protein
• dJ447F3.2
• EC 6.3.2.19
• UbcH10
• UBCH10mitotic-specific ubiquitin-conjugating enzyme
• UBE2C
• Ubiquitin carrier protein C
• ubiquitin carrier protein E2-C
• ubiquitin-conjugating enzyme E2 C
• ubiquitin-conjugating enzyme E2C
• Ubiquitin-protein ligase C

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for UbcH10/UBE2C Antibody (NBP2-20782)(1)

Publication using NBP2-20782

• Xiao Z, Shi G, Xi S et al. A TNFR1-UBCH10 axis drives lung squamous cell carcinoma dedifferentiation and metastasis through a cell-autonomous signaling loop Cell death & disease 2022-10-21 [PMID: 36270982] (Mouse)

Bioinformatics

• Gene Symbol: UBE2C
• Uniprot:
  • O00762(Human)