Recombinant Human His6UBE2S Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human His6UBE2S Protein, CF Summary

• Additional Information: Will be discontinued when existing inventory is gone.
• Details of Functionality: Recombinant Human His6-UBE2S is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2S concentration of 0.1-1 μM.
• Source: E. coli-derived human UBE2S protein
  Met1 - Leu222 with a N-terminal 6-His tag
• Accession #: NP_055316.2
• Protein/Peptide Type: Recombinant Enzymes
• Gene: UBE2S
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6UBE2S Protein, CF

• E2-24K
• E2-EPF
• E2-EPF5
• E2-EPF52
• E2-EPFEC 6.3.2.19
• E2EPFEPF5
• EPF5
• UBE2S
• Ubiquitin carrier protein S
• ubiquitin-conjugating enzyme E2 S
• ubiquitin-conjugating enzyme E2-24 kD
• Ubiquitin-conjugating enzyme E2-24 kDa
• Ubiquitin-conjugating enzyme E2-EPF5
• ubiquitin-conjugating enzyme E2S
• Ubiquitin-protein ligase S

Background

Ubiquitin-conjugating Enzyme E2S (UBE2S), also known as E2-EPF, is a widely expressed protein with a predicted molecular weight of 26 kDa (1). Human UBE2S shares 92% and 93% amino acid sequence identity with mouse and rat UBE2S, respectively. UBE2S expression oscillates in a cell cycle-dependent fashion, and it influences cell cycle progression by mediating the Ubiquitin-dependent proteolysis of mitotic regulators (2-4). UBE2S interacts with APC/C, a Ubiquitin ligase (E3), to catalyze the elongation of Lys11-linked Ubiquitin chains on APC/C substrates that have been mono-ubiquitinated by UbcH10/UBE2C or UbcH5/UBE2D (3-7). UBE2S is overexpressed in multiple human cancers and targets the tumor suppressor pVHL for proteasomal degradation, which stabilizes HIF-1 alpha and promotes tumor progression (8).

1. Liu, Z. et al. (1992) J. Biol. Chem. 267:15829.
2. Tedesco, D. et al. (2007) Neoplasia 9:601.
3. Williamson, A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:18213.
4. Dimova, N.V. et al. (2012) Nat. Cell Biol. 14:168.
5. Garnett, M.J. et al. (2009) Nat. Cell Biol. 11:1363.
6. Wu, T. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1355.
7. Bremm, A. & D. Komander (2012) Methods Mol. Biol. 832:219.
8. Jung, C.R. et al. (2006) Nat. Med. 12:809.

Publications for UBE2S (E2-690)(4)

Publications using E2-690

• OM Khan, J Almagro, JK Nelson, S Horswell, V Encheva, KS Keyan, BE Clurman, AP Snijders, A Behrens Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12 Nature Communications, 2021-04-06;12(1):2043. 2021-04-06 [PMID: 33824312] (Bioassay, Human)
• F Li, P Yuan, M Rao, CH Jin, W Tang, YF Rong, YP Hu, F Zhang, T Wei, Q Yin, T Liang, L Wu, J Li, D Li, Y Liu, W Lou, S Zhao, MF Liu piRNA-independent function of PIWIL1 as a co-activator for anaphase promoting complex/cyclosome to drive pancreatic cancer metastasis Nat. Cell Biol., 2020-03-16;0(0):. 2020-03-16 [PMID: 32203416] (Bioassay, Human)
• M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase Nat. Chem. Biol., 2019-02-15;15(3):276-284. 2019-02-15 [PMID: 30770915] (Enzyme Assay, Human)
• Neddylation E2 UBE2F promotes the survival of lung cancer cells by activating CRL5 to degrade NOXA via the K11 linkage Clin Cancer Res, 2016-09-02;0(0):. 2016-09-02 [PMID: 27591266] (Ubiquitination, Human)

Bioinformatics

• Gene Symbol: UBE2S
• Uniprot:
  • NP_055316.2()