UBAC1 Antibody - BSA Free

Western Blot: UBAC1 Antibody [NBP1-81842] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: UBAC1 Antibody [NBP1-81842] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.

Immunohistochemistry-Paraffin: UBAC1 Antibody [NBP1-81842] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Western Blot: UBAC1 Antibody [NBP1-81842] - Analysis in human cell line HDLM-2.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBAC1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MEMGFDEKEVIDALRVNNNQQNAACEWLLGDRKPSPEELDKGIDPDSPLFQAILDNPVVQLGLTNPKTLLAFEDMLENPLNSTQWMNDPETGPVMLQISRIFQTLNRT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBAC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  UBAC1 Protein (NBP1-81842PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for UBAC1 Antibody - BSA Free

• E3 ubiquitin-protein ligase subunit KPC2
• GBDR1UBA domain-containing protein 1
• Glialblastoma cell differentiation-related protein 1
• Kip1 ubiquitination-promoting complex protein 2
• KPC2
• putative glialblastoma cell differentiation-related protein
• RP11-432J22.3
• UBA domain containing 1
• UBADC1
• ubiquitin associated domain containing 1
• ubiquitin-associated domain-containing protein 1

Background

Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBAC1