U11/U12-35K Antibody - Azide and BSA Free

Western Blot: U11/U12-35K Antibody [NBP2-93775] - Analysis of extracts of various cell lines, using U11/U12-35K at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

U11/U12-35K Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit U11/U12-35K Antibody - Azide and BSA Free (NBP2-93775) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNRNP35 (NP_073208.1). MNDWMPIAKEYDPLKAGSIDGTDEDPHDRAVWRAMLARYVPNKGVIGDPLLTLFVARLNLQTKEDKLKEVFSRYGDIRRLRLVRDLVTGFSKGYAFIEYKEERAVIKAYRDADGLVIDQHEIFVDYELERTLKGWIPRRLGGGLGGKKES
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNRNP35
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for U11/U12-35K Antibody - Azide and BSA Free

• HM1
• HM-1
• MGC138160
• Protein HM-1
• small nuclear ribonucleoprotein 35kDa (U11/U12)
• U1 snRNP-binding protein homolog
• U11/U12 small nuclear ribonucleoprotein 35 kDa protein
• U11/U12 snRNP 35 kDa protein
• U11/U12 snRNP 35K
• U11/U12-35K
• U1-snRNP binding protein homolog
• U1SNRNPBPU1 snRNP binding protein homolog

Background

The protein encoded by the U1SNRNPBP gene is a homolog of U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal is rich in Arg/Asp and Arg/Glu dipeptides; a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. This gene is differentially expressed in a variety of human tissues. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: SNRNP35