U11/U12-35K Antibody

Images

 
Western Blot: U11/U12-35K Antibody [NBP2-20774] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: U11/U12-35K Antibody [NBP2-20774] - U11/U12-35K antibody detects U11/U12-35K protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% ...read more
Immunohistochemistry-Paraffin: U11/U12-35K Antibody [NBP2-20774] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
Western Blot: U11/U12-35K Antibody [NBP2-20774] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

U11/U12-35K Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human U1SNRNPBP. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNRNP35
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for U11/U12-35K Antibody

  • HM1
  • HM-1
  • MGC138160
  • Protein HM-1
  • small nuclear ribonucleoprotein 35kDa (U11/U12)
  • U1 snRNP-binding protein homolog
  • U11/U12 small nuclear ribonucleoprotein 35 kDa protein
  • U11/U12 snRNP 35 kDa protein
  • U11/U12 snRNP 35K
  • U11/U12-35K
  • U1-snRNP binding protein homolog
  • U1SNRNPBPU1 snRNP binding protein homolog

Background

The protein encoded by this gene is a homolog of U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal is rich in Arg/Asp and Arg/Glu dipeptides; a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. This gene is differentially expressed in a variety of human tissues. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SNRNP35
Uniprot