TWEAK/TNFSF12 Antibody (CARL-1)

Product Discontinued

TWEAK/TNFSF12 Antibody (CARL-1) Summary

• Immunogen: hTWEAK/2PK-3 transfectant cells
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF12
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1-4 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: IHC and Neutralization use reported in literature. Use in WB and ICC/IF was reported in the scientific literature (PMID: 21788066)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for TWEAK/TNFSF12 Antibody (CARL-1)

• APO3 ligand
• APO3LMGC20669
• DR3LGTWEAKAPO3/DR3 ligand
• MGC129581
• TNF-related weak inducer of apoptosis
• TNFSF12
• tumor necrosis factor (ligand) superfamily, member 12
• tumor necrosis factor ligand superfamily member 12
• TWEAK

Background

TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF- kappaB, proliferation of endothelial cells, and apoptosis in a number of human cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF12
• Entrez:
  • 8742(Human)
• Uniprot:
  • O43508()