Tubulin Beta 4 Antibody (ONS.1A6)

Western Blot: Tubulin Beta 4 Antibody (ONS.1A6) [NB120-11315] - MCF7 Human breast carcinoma cells extract was separated on SDS-PAGE and probed with Monoclonal AB To Tubulin Beta IV CL:ONS.1A6. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/400.

Product Discontinued

Tubulin Beta 4 Antibody (ONS.1A6) Summary

• Immunogen: Synthetic peptide corresponding to the C-terminal sequence, conjugated to BSA.
• Localization: Centrosomal
• Specificity: The does not react with other tubulin isotypes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB4A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Microarray
  • Western Blot 1:400
• Application Notes: Western Blot. WB: Detects a band of approximately 55 kDa and possibly the 100 kDa dimer.
• Theoretical MW: ~55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Tubulin Beta 4 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Cow and Chicken. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Tubulin Beta 4 Antibody (ONS.1A6)

• TUBB4
• TUBB4A
• TUBB5beta-5
• Tubulin 5 beta
• Tubulin Beta 4
• tubulin beta-4 chain
• tubulin, beta 4
• tubulin, beta, 5

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tubulin Beta 4 Antibody (NB120-11315)(2)

Publications using NB120-11315

• Radwitz J, Hausrat TJ, Heisler FF et al. Tubb3 expression levels are sensitive to neuronal activity changes and determine microtubule growth and kinesin-mediated transport Cellular and molecular life sciences : CMLS 2022-10-29 [PMID: 36309617] (ICC/IF)
• Hausrat TJ, Radwitz J, Lombino FL et al. Alpha- and beta-tubulin isotypes are differentially expressed during brain development Dev Neurobiol 2020-04-15 [PMID: 32293117] (WB, Human)

Bioinformatics

• Gene Symbol: TUBB4A
• Entrez:
  • 10382(Human)
  • 22153(Mouse)
  • 29213(Rat)