Tubulin Beta 2C Antibody (ST52-04)

Western Blot: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Analysis of beta II Tubulin on different lysates using anti-beta II Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Mouse kidney

Immunocytochemistry/Immunofluorescence: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Staining beta II Tubulin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tubulin Beta 2C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Analysis of paraffin-embedded human kidney tissue using anti-beta II Tubulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Analysis of paraffin-embedded mouse brain tissue using anti-beta II Tubulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Tubulin Beta 2C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Tubulin Beta 2C Antibody (ST52-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Tubulin Beta 2C aa 407-445 / 445. (SwissProt: P68371 Human; SwissProt: Q13885 Human; SwissProt: Q9BVA1 Human; SwissProt: P68372 Mouse; SwissProt: Q7TMM9 Mouse; SwissProt: Q9CWF2 Mouse; SwissProt: P85108 Rat; SwissProt: Q3KRE8 Rat; SwissProt: Q6P9T8 Rat)
• Localization: Cytoplasm, Cytoskeleton, Microtubule.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUBB4B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Tubulin Beta 2C Antibody (ST52-04)

• class IVb beta tubulin
• TUBB2
• TUBB2C
• TUBB4B
• Tubulin Beta 2C
• Tubulin beta-2 chain
• tubulin beta-2C chain
• tubulin, beta 2C
• tubulin, beta, 2

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB4B