TTF-1 / NKX2-1 Antibody (EP1584Y)

Product Discontinued

TTF-1 / NKX2-1 Antibody (EP1584Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human TTF1 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NKX2-1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:30 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:400
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Western Blot 1:500 - 1:1000
• Application Notes: Useful in Immunohistochemistry-Paraffin (PMID: 21364949)
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.05% BSA, 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for TTF-1 / NKX2-1 Antibody (EP1584Y)

• BCH
• benign chorea
• BHC
• Homeobox protein NK-2 homolog A
• homeobox protein Nkx-2.1
• NK2 homeobox 1
• NK-2 homolog A
• NKX2.1
• NKX2-1
• NKX2A
• NKX2ANKX2.1
• T/EBP
• Thyroid nuclear factor 1
• Thyroid transcription factor 1BHC
• TITF1
• TITF1TEBP
• TTF1
• TTF-1
• TTF1BCH
• TTF-1NK-2

Background

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TTF-1 / NKX2-1 Antibody (NB100-80062)(7)

Publications using NB100-80062

• Sakurai T, Isogaya K, Sakai S et al. RNA-binding motif protein 47 inhibits Nrf2 activity to suppress tumor growth in lung adenocarcinoma. Oncogene 2016-01-01 [PMID: 26923328] (WB, Mouse)
• Leong TL, Marini KD, Rossello FJ et al. Genomic characterisation of small cell lung cancer patient-derived xenografts generated from endobronchial ultrasound-guided transbronchial needle aspiration specimens. PLoS ONE. 2014-09-06 [PMID: 25191746]
• Miller A, Brooks GD, McLeod L et al. Differential involvement of gp130 signalling pathways in modulating tobacco carcinogen-induced lung tumourigenesis. Oncogene 2014-04-14 [PMID: 24727895] (IHC-P, Mouse)
• Micciche F, Da Riva L, Fabbi M et al. Activated leukocyte cell adhesion molecule expression and shedding in thyroid tumors. PLoS ONE;6(2). 2011-01-01 [PMID: 21364949]
• Yu X, Zecevic N et al. Dorsal radial glial cells have the potential to generate cortical interneurons in human but not in mouse brain. J Neurosci 2011-02-01 [PMID: 21325508]
• Doles J, Oliver TG, Cameron ER et al. Suppression of Rev3, the catalytic subunit of Pol?, sensitizes drug-resistant lung tumors to chemotherapy . Proc Natl Acad Sci U S A 107 20786-10791. 2010-01-01 [PMID: 21068376]
• Yu W, Wang Y, McDonnell K et al. Patterning of ventral telencephalon requires positive function of Gli transcription factors. Dev Biol 2009-10-01 [PMID: 19632216] (IF/IHC)

Review for TTF-1 / NKX2-1 Antibody (NB100-80062) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/19/2014

Summary

• Application: Western Blot
• Sample Tested: WB for NKX2.1 in MOLT-4 cells
• Species: Human

Bioinformatics

• Gene Symbol: NKX2-1
• Entrez:
  • 7080(Human)
  • 22130(Mouse)
  • 499766(Rat)
• Uniprot:
  • P43699()