TSPAN9 Antibody (590409)

Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TSPAN9 Monoclonal Antibody (Catalog # MAB6565) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TSPAN9 at approximately 27 to 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 590409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TSPAN9 Antibody (590409) Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human TSPAN9
  Accession # O75954
• Specificity: Detects human TSPAN9 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSPAN9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN9 Antibody (590409)

• NET5
• NET-5
• NET5tetraspanin-9
• new EST tetraspan 5
• PP1057
• Tetraspan NET-5
• tetraspanin 9
• transmembrane 4 superfamily member tetraspan NET-5
• TSPAN9
• tspan-9

Background

Tetraspanin 9 (TSPAN9) is a 27 kDa glycoprotein and member of the tetraspanin (TM4SF) family of proteins. It is 239 amino acids (aa) in length and contains four transmembrane segments and one potential site for N-linked glycosylation. Both the N- and C-termini lie on the intracellular side of the membrane. Human TSPAN9 is 97% aa identical to mouse TSPAN9. It is expressed in megakaryocytes and platelets where it co-localizes with GP6 in tetraspanin microdomains on the platelet surface. TSPAN9 is involved in platelet activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSPAN9
• Uniprot:
  • O75954()