TSPAN18 Overexpression Lysate

Images

 
Western Blot: TSPAN18 Overexpression Lysate (Adult Normal) [NBL1-17371] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TSPAN18.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TSPAN18 Overexpression Lysate Summary

Description

TSPAN18 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202968

Accession#: NM_001031730

Protein Tag: C-MYC/DDK
Gene
TSPAN18

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TSPAN18 Overexpression Lysate

  • tetraspanin 18
  • TSPAN
  • TSPAN18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF942
Species: Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, WB
NB100-689
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-67415
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-24583
Species: Bv, Eq, Hu, Mu, Ma-Op, Pm, Rt
Applications: IHC, IHC-P, WB
H00387032-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
7268-CT
Species: Hu
Applications: BA
NB500-201
Species: Ca, Fe, Gp, Ha, Hu, Mu, Rt
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NBP2-59438
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP1-80656
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-59438
Species: Hu
Applications: IHC, WB
DVE00
Species: Hu
Applications: ELISA
NB600-1182
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAB7089
Species: Mu
Applications: CyTOF-ready, Flow, IHC
NB100-56519
Species: Bv, Hu, Mu, Po, Rt, Sh
Applications: ChIP, CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
H00005307-M01
Species: Hu
Applications: ChIP, ELISA, IHC, IHC-P, IP, KD, S-ELISA, WB
H00001351-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NB100-2737
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-32550
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for TSPAN18 Lysate (NBL1-17371) (0)

There are no publications for TSPAN18 Lysate (NBL1-17371).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TSPAN18 Lysate (NBL1-17371) (0)

There are no reviews for TSPAN18 Lysate (NBL1-17371). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TSPAN18 Lysate (NBL1-17371) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TSPAN18 Products

Bioinformatics Tool for TSPAN18 Lysate (NBL1-17371)

Discover related pathways, diseases and genes to TSPAN18 Lysate (NBL1-17371). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TSPAN18 Lysate (NBL1-17371)

Discover more about diseases related to TSPAN18 Lysate (NBL1-17371).
 

Pathways for TSPAN18 Lysate (NBL1-17371)

View related products by pathway.

Blogs on TSPAN18

There are no specific blogs for TSPAN18, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TSPAN18 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol TSPAN18