TSPAN12 Antibody (921938) [Alexa Fluor® 647]

HEK293 human embryonic kidney cell line transfected with (A) human TSPAN12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN12 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB8910R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 921938
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

TSPAN12 Antibody (921938) [Alexa Fluor® 647] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human TSPAN12
  Met1-Leu305
  Accession # O95859
• Specificity: Stains human TSPAN12 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSPAN12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN12 Antibody (921938) [Alexa Fluor® 647]

• EVR5
• Leukemia-associated protein with a CXXC domain
• methylcytosine dioxygenase TET1
• NET2
• NET-2
• ten-eleven translocation-1
• tetraspanin-12
• TM4SF12
• TSPAN12

Background

TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. They mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN12 plays a central role in retinal vascularization by regulating norrin signal transduction. TSPAN12 acts in concert with norrin to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin and stimulate LEF/TCF-mediated transcriptional programs. Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5, a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSPAN12 Antibody (FAB8910R)(1)

Publication using FAB8910R

• Granados, AJ;Pratap, PP;Alamo, KAE;Susa, KJ; Structural basis for regulation of Frizzled-4 signaling by the co-receptor Tetraspanin-12 bioRxiv : the preprint server for biology 2025-09-25 [PMID: 41040411] (Flow Cytometry, Immunocytochemistry, Human)

Bioinformatics

• Gene Symbol: TSPAN12
• Uniprot:
  • O95859(Human)