Home » TSLPR/CRLF2 » TSLPR/CRLF2 Antibodies » TSLPR/CRLF2 Antibody [Unconjugated]

TSLPR/CRLF2 Antibody [Unconjugated]

 (5 Publications)
 (2 Reviews)
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Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TSLP R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG ...read more
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified ...read more
TSLP R was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 5 µg/mL overnight at 4 °C. Tissue ...read more
Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R in a dose-dependent manner (orange line). Proliferation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF981
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Catalog# & Formulation Size Price
Carrier Free
AF981-SP
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TSLPR/CRLF2 Antibody [Unconjugated] Summary

Specificity
Detects human TSLP R in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRLF2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
  • Western Blot 0.2-1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF981 in the following applications:
Publications
Read Publications using
AF981 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSLPR/CRLF2 Antibody [Unconjugated]

  • CRL2
  • CRL2cytokine receptor CRL2 precusor
  • CRLF2
  • CRLF2Y
  • CRLM-2
  • Cytokine receptor-like 2
  • cytokine receptor-like factor 2
  • ILXR
  • IL-XR
  • P2RY8/CRLF2 fusion
  • PCOR1
  • Thymic stromal lymphopoietin protein receptor
  • thymic stromal lymphopoietin receptor
  • thymic stromal-derived lymphopoietin receptor
  • TSLP R
  • TSLP receptor
  • TSLPR

Background

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS‑like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell‑attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).

  1. Fujio, K. et al. (2000) Blood 95:2204.
  2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
  5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TSLPR/CRLF2
Antibody [Unconjugate
AF981
TSLPR/CRLF2 Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, B/N, ICC/IF

Publications for TSLPR/CRLF2 Antibody (AF981)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IHC-Pr, Neutralization, Western Blot.


Filter By Application
IHC
(1)
IHC-Pr
(1)
Neutralization
(2)
Western Blot
(1)
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Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using AF981 Applications Species
SZ Li, XX Jin, XL Ge, YG Zuo, HZ Jin Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells J Immunol Res, 2020;2020(0):4594630. 2020 [PMID: 33029540] (IHC, Human) IHC Human
R Ohe, NY Aung, Y Shiono, A Utsunomiya, T Kabasawa, N Tamazawa, Y Tamura, T Kato, A Yamada, S Hasegawa, K Aizawa, K Inokura, S Ito, T Toubai, Y Kato, T Tsunoda, K Onami, T Suzuki, K Ishizawa, M Yamakawa Detection of Minimal Bone Marrow involvement of Blastic Plasmacytoid Dendritic Cell Neoplastic Cells - CD303 immunostaining as a diagnostic tool J Clin Exp Hematop, 2018;0(0):. 2018 [PMID: 29415975] (IHC-Pr, Human) IHC-Pr Human
B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils Oncol Lett, 2017;14(6):7483-7488. 2017 [PMID: 29344192] (Neutralization, Human) Neutralization Human
Y Peng, K Meng, L Jiang, Y Zhong, Y Yang, Y Lan, Q Zeng, L Cheng Thymic stromal lymphopoietin-induced HOTAIR activation promotes endothelial cell proliferation and migration in atherosclerosis Biosci. Rep., 2017;37(4):. 2017 [PMID: 28615347] (Neutralization, Human) Neutralization Human
Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Blood, 2010;116(12):2061-9. 2010 [PMID: 20538796] (Western Blot, Human) Western Blot Human

Reviews for TSLPR/CRLF2 Antibody (AF981) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF981:
Filter by Applications
Western Blot
(1)
Flow Cytometry
(1)
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Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
cynthia morimoto
 Western Blot Human 01/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20624642
SpeciesHuman
  4
reviewed by:
cynthia morimoto
 Flow Cytometry Human 01/12/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20624642
SpeciesHuman

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Recent Reviews

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 cynthia morimoto
01/12/2015
Application: Western Blot
Species: Human
read this review
 
 cynthia morimoto
01/12/2015
Application: Flow Cytometry
Species: Human
read this review

Bioinformatics

Gene Symbol CRLF2
Uniprot