Reactivity | HuSpecies Glossary |
Applications | WB, IHC, B/N, ICC/IF |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Specificity | Detects human TSLP R in direct ELISAs and Western blots. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | CRLF2 |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS‑like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell‑attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).
Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
cynthia morimoto |
Western Blot | Human | 01/12/2015 |
Summary
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reviewed by:
cynthia morimoto |
Flow Cytometry | Human | 01/12/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for TSLPR/CRLF2 Antibody (AF981)Discover more about diseases related to TSLPR/CRLF2 Antibody (AF981).
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PTMs for TSLPR/CRLF2 Antibody (AF981)Learn more about PTMs related to TSLPR/CRLF2 Antibody (AF981).
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cynthia morimoto 01/12/2015 |
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Application: | Western Blot | |
Species: | Human |
cynthia morimoto 01/12/2015 |
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Application: | Flow Cytometry | |
Species: | Human |