TSLPR/CRLF2 Antibody [Unconjugated]

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TSLP R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TSLP R was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.3 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981). The ND50 is typically 1‑5 µg/mL.

• Reactivity: Hu
• Applications: WB, IHC, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TSLPR/CRLF2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TSLP R
  Val30-Phe232
  Accession # Q9HC73
• Specificity: Detects human TSLP R in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRLF2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2-1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSLPR/CRLF2 Antibody [Unconjugated]

• CRL2
• CRL2cytokine receptor CRL2 precusor
• CRLF2
• CRLF2Y
• CRLM-2
• Cytokine receptor-like 2
• cytokine receptor-like factor 2
• ILXR
• IL-XR
• P2RY8/CRLF2 fusion
• PCOR1
• Thymic stromal lymphopoietin protein receptor
• thymic stromal lymphopoietin receptor
• thymic stromal-derived lymphopoietin receptor
• TSLP R
• TSLP receptor
• TSLPR

Background

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS‑like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell‑attracting chemokines from monocytes and enhance the maturation of CD11c⁺ dendritic cells (5).

1. Fujio, K. et al. (2000) Blood 95:2204.
2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSLPR/CRLF2 Antibody (AF981)(4)

Publications using AF981

• R Ohe, NY Aung, Y Shiono, A Utsunomiya, T Kabasawa, N Tamazawa, Y Tamura, T Kato, A Yamada, S Hasegawa, K Aizawa, K Inokura, S Ito, T Toubai, Y Kato, T Tsunoda, K Onami, T Suzuki, K Ishizawa, M Yamakawa Detection of Minimal Bone Marrow involvement of Blastic Plasmacytoid Dendritic Cell Neoplastic Cells - CD303 immunostaining as a diagnostic tool J Clin Exp Hematop, 2018;0(0):. 2018 [PMID: 29415975] (IHC-Pr, Human)
• B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils Oncol Lett, 2017;14(6):7483-7488. 2017 [PMID: 29344192] (Neutralization, Human)
• Y Peng, K Meng, L Jiang, Y Zhong, Y Yang, Y Lan, Q Zeng, L Cheng Thymic stromal lymphopoietin-induced HOTAIR activation promotes endothelial cell proliferation and migration in atherosclerosis Biosci. Rep., 2017;37(4):. 2017 [PMID: 28615347] (Neutralization, Human)
• Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Blood, 2010;116(12):2061-9. 2010 [PMID: 20538796] (Western Blot, Human)

Reviews for TSLPR/CRLF2 Antibody (AF981) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous WB Human 01/12/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 20624642
• Species: Human

Blocking

• Blocking Details: See PMID 20624642

Details

• Detection Notes: See PMID 20624642

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 20624642.

reviewed by: Anonymous Flow Human 01/12/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 20624642
• Species: Human

Blocking

• Blocking Details: See PMID 20624642

Details

• Detection Notes: See PMID 20624642

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 20624642

Bioinformatics

• Gene Symbol: CRLF2
• Uniprot:
  • Q9HC73()